Home / Guides / How to inject semaglutide
How-to · October 2026
How to inject compounded semaglutide from a vial, step by step
Medically reviewed by the NexLife clinical team, led by Adam Kennah, MD · Updated
Using a new U-100 insulin syringe, draw your prescribed units and inject under the skin of your stomach, thigh or upper arm, changing spots weekly. Units depend on your vial’s strength, so follow your label; NexLife answers vial questions 24/7 at
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- Your pharmacy label and your provider’s instructions come first
- At 2.5 mg/mL, 0.25 mg is 10 units; at 5 mg/mL, it’s 5 units
- Stomach, thigh or upper arm, a new spot each week
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Key takeaways
- Your pharmacy label and your provider come first. These steps follow the Wegovy label’s injection instructions where they fit a vial and syringe: a new syringe every time, a clean spot, and a new spot each week.
- Units depend on your vial’s strength. On a U-100 syringe, units = mg ÷ mg/mL × 100. So 10 units is 0.25 mg from a 2.5 mg/mL vial, but 0.5 mg from a 5 mg/mL vial.
- Stomach, thigh or upper arm, a new spot each week. Stay at least 2 inches from your belly button, and have someone help with the back of your arm.
- The FDA has had reports of adverse events, some needing hospital care, that may be linked to dosing errors with compounded semaglutide. If anything doesn’t match, ask before you inject.
- At NexLife, questions about your vial come to us, 24/7: call or text
(949) 818-8000 , and we’ll talk you through your first dose. Compounded semaglutide is $119 a month on the 12-month plan, or $139 month to month.
What you need
What do you need to inject semaglutide from a vial?
Six things, set out on a clean surface before you start. Wegovy’s instructions list the same basics for its pens and syringes.
Your vial
Check its label: the medicine’s name, its strength in mg per mL, its beyond-use date and the directions for your dose.
A new U-100 insulin syringe
One for every dose, needle attached, big enough to hold your units. Use the one your pharmacy or provider recommends.
Alcohol swabs
A fresh one for the vial’s rubber top every time, and one for your skin. Wegovy’s pen instructions also allow soap and water for the skin.
Gauze or a cotton ball
To press lightly on the spot if you see a little blood.
A sharps container
An FDA-cleared one, or a heavy-duty plastic household container with a tight-fitting, puncture-resistant lid.
Your prescription
Your dose in mg, and how many units that is with your vial.
Step by step
How to draw and inject semaglutide from a multi-dose vial
Wegovy comes in pens and prefilled syringes, not vials, and compounded semaglutide isn’t FDA-approved, so no FDA-approved instructions cover your vial. These steps follow the Wegovy label where it fits a vial and syringe. Your pharmacy label and your provider’s instructions come first.
Before you start
- Check the label. The right medicine, the strength you expect, and not past its beyond-use date or 28 days from your first dose out of it. It should say semaglutide, not semaglutide sodium or acetate.
- Look at the liquid. Don’t use it if it’s cloudy, has particles or has ever been frozen. Wegovy is clear and colorless; added vitamin B12 can tint a compounded vial pink or red, and color doesn’t tell you the strength.
- Wash and dry your hands.
Then, 10 steps
Wipe the rubber top
The first time, take off the plastic cap but leave the rubber stopper in place. Every time, wipe the top with a new alcohol swab and let it dry. Don’t blow on it.
Uncap a new syringe
Use a new needle and syringe for every dose. Pull the needle cap straight off, and don’t let the needle touch anything.
Pull in air to your dose
Pull the plunger back to your number of units (how many?), so the syringe holds that much air.
Push the air into the vial
Push the needle through the rubber top, then push the plunger in so the air goes into the vial.
Turn it over and draw
Keep the needle in and turn the vial upside down. With the needle tip in the liquid, pull the plunger back to your units line.
Check for bubbles
If you see bubbles, tap the syringe so they float up, push them back into the vial, then draw to your units line again. Check it against your prescription.
Take the needle out
Pull the syringe out of the vial; never leave a needle in the rubber top. Set the syringe down so the needle touches nothing.
Clean your spot
Pick a spot on your stomach, thigh or upper arm, away from last week’s. Clean it with an alcohol swab, or soap and water, and let it dry without touching it.
Pinch and inject
Pinch a fold of skin, fully insert the needle at the angle your provider showed you, and push the plunger all the way down.
Hold, then finish safely
Leave the needle in for about 5 seconds, then pull it out at the same angle. If you see blood, press lightly with gauze; don’t rub. Don’t recap the needle: the syringe goes straight into your sharps container.
Which angle? Wegovy’s prefilled-syringe instructions pinch the skin and insert the needle at 45 degrees, to stay under the skin and out of the muscle. NIH’s MedlinePlus guide to under-the-skin shots uses 90 degrees, or 45 if there isn’t much fatty tissue. Use the angle your provider showed you.
Where the steps come from. Drawing from the vial and the 5-second hold follow MedlinePlus’s steps for insulin syringes. Choosing, cleaning and finishing at the spot follow Wegovy’s instructions.
What’s different with a multi-dose vial
You measure the dose yourself
A Wegovy pen or prefilled syringe holds a measured dose. With a vial, your units depend on your dose and your vial’s strength.
You go back in every week
So it’s a new needle and syringe and a fresh swab on the rubber top every time, and no needle left in the top between doses.
Date it the first time
Write the date of your first dose on the vial or its box. The FDA says to throw it out within 28 days of first use, even with medicine left, and even if the compounder’s instructions say longer.
A new vial can mean new units
Check the strength on every new vial’s label. The same dose can be a different number of units.
Not sure about a step? Ask before you inject.
Call or text NexLife at
Units
How many units of semaglutide do you draw?
It depends on your vial’s strength. On a U-100 insulin syringe, 100 units is 1 mL, so units = your dose in mg ÷ your vial’s strength in mg/mL × 100.
| Dose | 1 mg/mL | 2.5 mg/mL | 5 mg/mL |
|---|---|---|---|
| 0.25 mg | 25 units | 10 units | 5 units |
| 0.5 mg | 50 units | 20 units | 10 units |
| 1 mg | 100 units | 40 units | 20 units |
| 1.7 mg | Over 1 mL | 68 units | 34 units |
| 2.4 mg | Over 1 mL | 96 units | 48 units |
Swipe the table sideways to see every column.
Example only: follow your label and your prescription. Units on a U-100 insulin syringe (100 units = 1 mL) for the step-up doses on the Wegovy label, at three example vial strengths. “Over 1 mL” won’t fit in a 1 mL syringe.
10 units on a U-100 syringe is 0.1 mL. How much medicine that holds depends on the vial’s strength, so give your provider the milligrams on your label, not the units.
Got a fraction? At 3 mg/mL, for example, 0.25 mg is about 8.3 units, which is hard to measure on a syringe marked in whole or half units. Ask your provider or pharmacy exactly what to draw before you inject.
Need another dose or strength? Our semaglutide dosage chart has every step-up dose at five strengths, and a converter for any vial.
Syringe size
Which syringe size do you need for semaglutide?
One that holds your whole dose in a single draw. On a U-100 insulin syringe, the size in mL sets how many units fit.
| Syringe | Holds | At 2.5 mg/mL, up to | At 5 mg/mL, up to |
|---|---|---|---|
| 0.3 mLU-100 | 30 units | 0.75 mg | 1.5 mg |
| 0.5 mLU-100 | 50 units | 1.25 mg | 2.5 mg |
| 1 mLU-100 | 100 units | 2.5 mg | 5 mg |
Swipe the table sideways to see every column.
Example only: use the syringe your pharmacy or provider recommends. On a U-100 insulin syringe, 100 units = 1 mL; the last two columns are the largest dose that fits in one draw.
Small doses? Some 0.3 mL syringes are marked in half units, which makes a dose like 12.5 units (0.25 mg at 2 mg/mL) easier to read. Whatever you use, check what each line stands for before you draw.
The needle. MedlinePlus says needles for under-the-skin shots are very short and thin. Use a new one every time: the CDC’s rule is one needle, one syringe, only one time.
Where to inject
Where to inject semaglutide, and how to rotate
Under the skin of your stomach, thigh or upper arm, the three areas on the Wegovy label. Change the spot with every dose, even if you stay in the same area.
Stomach
Your lower stomach, at least 2 inches from your belly button.
Thigh
The front of your thigh. Easy to see when you inject yourself.
Upper arm
The back of your upper arm. Wegovy’s syringe instructions say to use it only with help from someone else.
A rotation map: four spots, one a week
- Week 1Stomach, left of your belly button
- Week 2Stomach, right of your belly button
- Week 3Left thigh
- Week 4Right thigh
Then start again, at least an inch from the spot you used last time in that area. Have a helper? Add the back of each upper arm. A note on your phone with the date and the spot makes the next one easy.
Does the area matter? The Wegovy label says similar levels of semaglutide reached the blood from the stomach, thigh or upper arm, and that you can change the site without changing the dose.
- Skip skin that’s tender, bruised, red or hard, and areas with scars or stretch marks.
- Same area, new spot. Wegovy’s instructions allow the same body area each week, but not the same spot.
- Under the skin only, never into a muscle or a vein.
- On insulin too? Give them as separate injections, and never mix them in one syringe. The Ozempic label allows the same area, but not right next to each other.
After injecting
After your injection: what’s normal, and when to call
A little soreness where you injected is expected, NIH’s MedlinePlus says. Here’s what the Wegovy label lists, when to get help, and how to throw the syringe away.
How common are skin reactions? In Wegovy’s weight-loss trials in adults, 1.4% of people on Wegovy had an injection-site reaction, against 1% on placebo: itching, redness, inflammation, hardening or irritation where they injected. These trials studied FDA-approved Wegovy; compounded semaglutide wasn’t studied.
| What you notice | What to do |
|---|---|
| A little blood as the needle comes out | Press lightly with gauze or a cotton ball. Don’t rub it. |
| Soreness, a small bump, redness or itching where you injected | Some soreness is expected. Keep changing spots, and tell your provider if it bothers you or doesn’t go away. |
| Signs of an infection, such as redness, swelling, red streaks and a fever | Call your provider. |
| Severe stomach pain that won’t go away with or without nausea or vomiting | Stop semaglutide and call your provider right away. It can be a sign of pancreatitis. |
| Signs of a serious allergic reaction: swelling of your face, lips, tongue or throat; problems breathing or swallowing; a severe rash or itching; fainting or dizziness; a very rapid heartbeat | Stop semaglutide and get medical help right away: call 911. |
Swipe the table sideways to see every column.
From the Wegovy Medication Guide and instructions for use (signs of infection from NIH’s MedlinePlus). Your provider’s instructions come first.
Throwing away the syringe
- Right away, put the used syringe in an FDA-cleared sharps container. No container? Use a heavy-duty plastic household container with a tight-fitting, puncture-resistant lid that stays upright, resists leaks and is labeled as hazardous waste.
- About three-quarters full? Follow your community’s rules. Drop-off sites can include pharmacies, doctors’ offices, hospitals and health departments, and some areas have mail-back programs.
- Not in the trash or recycling, unless your community’s rules allow the trash. Don’t reuse the container.
- Not sure what your area allows? The FDA points to Safe Needle Disposal: 1-800-643-1643.
Then put the vial away. Our semaglutide storage guide covers the fridge, the 28-day rule and what to do if a delivery arrives warm.
Avoid these
Common mistakes with semaglutide vials
Most come down to units, needles and storage. The FDA has had reports of adverse events, some needing hospital care, that may be linked to people measuring the wrong dose of compounded semaglutide.
- Mixing up mL and units. On a U-100 syringe, 0.1 mL is 10 units, not 1. Your dose is in mg, your syringe counts units, and your vial’s strength links the two.
- Copying units from an old vial. A new vial or a new pharmacy can mean a new strength: 0.25 mg is 10 units at 2.5 mg/mL but 5 units at 5 mg/mL. Read the label on every new vial.
- Reusing a needle or syringe. Even on yourself. The FDA warns it can contaminate the medicine in your vial, and Wegovy’s pen instructions say a new needle each time prevents infection, leaks and blocked needles that lead to the wrong dose.
- Sharing needles or syringes. Never, even with family. The Wegovy Medication Guide warns that you can give someone a serious infection, or get one.
- Injecting into a muscle. Semaglutide goes under the skin, never into a muscle or a vein. Pinching a fold of skin helps.
- Leaving a needle in the rubber top. The CDC warns that it gives germs a direct route into the vial. Take it out every time.
- Using a vial that froze or arrived warm. Don’t, even if a frozen vial has thawed. Keep it and the box, and contact the program that prescribed it.
- Going past day 28. Throw out an opened vial no later than 28 days after its first use, even with medicine left, or sooner if its label says so.
- Mixing it with insulin. The Ozempic label says to give semaglutide and insulin as separate injections and never to mix them.
- Recapping a needle, or tossing it in the trash. Recapping can stick you. Put the syringe straight into a sharps container, never loose in the trash or recycling.
Think you injected too much? You may have severe nausea, severe vomiting and severe low blood sugar. Call your provider or Poison Help at 1-800-222-1222, or go to the nearest emergency room right away, as the Wegovy Medication Guide says.
At NexLife
Questions about your vial? Call or text us, 24/7
Questions about your vial, your units or your label come to us, not the pharmacy. Real people answer at
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Text us a photo of the label. We check with your provider and the pharmacy, and get back to you before your next dose.
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Don’t use it. Keep the vial and the box, and call or text us. We contact the pharmacy for you.
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We get your question to your provider. Physicians answer medical questions during clinical hours.
New to semaglutide? Start with a short online assessment, about 3 minutes. A licensed provider reviews it and prescribes only if treatment is right for you. Compounded semaglutide is $119 a month on the 12-month plan, or $139 month to month, the same at every dose.
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Injecting semaglutide: your questions
How do you inject semaglutide from a vial?
Using a new U-100 insulin syringe, draw your prescribed units and inject under the skin of your stomach, thigh or upper arm, changing spots weekly. Wipe the vial’s rubber top first, push in air equal to your dose, check for bubbles, and put the used syringe straight into a sharps container. Your pharmacy label and your provider’s instructions come first.
Where is the best place to inject semaglutide?
The Wegovy label lists your stomach, thigh or upper arm, and says similar levels of semaglutide reached the blood from all three. Stay at least 2 inches from your belly button and use a new spot each week. Wegovy’s syringe instructions say to use the back of your arm only with help.
How many units is 0.25 mg of semaglutide?
It depends on your vial’s strength. At 2.5 mg/mL, 0.25 mg is 10 units on a U-100 syringe; at 5 mg/mL, it’s 5 units; at 1 mg/mL, it’s 25 units. Your prescription and your vial’s label set your dose, so follow them.
What size syringe do I need for semaglutide?
A U-100 insulin syringe that holds your whole dose: a 0.3 mL syringe holds 30 units, 0.5 mL holds 50 and 1 mL holds 100. Some 0.3 mL syringes have half-unit marks, which help with small doses. Use the one your pharmacy or provider recommends, and a new one every time.
Can I reuse a syringe?
No. Use a new needle and syringe for every dose, even on yourself: the FDA says reusing them can contaminate the medicine in your vial. Never share needles or syringes, either. The Wegovy Medication Guide warns you can give someone a serious infection, or get one.
Should I pinch the skin?
Wegovy’s prefilled-syringe instructions pinch a fold of skin and hold it through the injection, so the needle goes under the skin, not into muscle. NIH’s MedlinePlus pinches about an inch of skin and fat. Follow what your provider showed you; at NexLife, call or text
What angle should the needle go in?
The angle your provider showed you. Wegovy’s prefilled-syringe instructions use 45 degrees. NIH’s MedlinePlus guide to under-the-skin shots, which uses very short needles, says 90 degrees, or 45 if there isn’t much fatty tissue.
What if I see blood or a bump after injecting?
A little blood can happen: press lightly with gauze or a cotton ball, without rubbing. Some soreness is expected, and a small bump, redness or itching can be an injection-site reaction. Call your provider about signs of an infection, such as swelling, red streaks and a fever, and 911 for swelling of your face or throat or trouble breathing.
How do I throw away used syringes?
Put each one straight into an FDA-cleared sharps container, or a heavy-duty plastic household container with a tight, puncture-resistant lid. Don’t recap the needle. When it’s about three-quarters full, follow your community’s rules; drop-off sites can include pharmacies, doctors’ offices and health departments.
Do I need to wipe my skin with alcohol?
Wegovy’s instructions say to clean the spot with an alcohol swab, or with soap and water, then let it dry without touching it. Wipe the vial’s rubber top with a fresh alcohol swab too, and let it dry before the needle goes in.
Important safety information
Compounded tirzepatide and semaglutide are not FDA-approved, and the FDA hasn’t reviewed them for safety, effectiveness or quality. A provider prescribes them only when they offer a significant difference for you compared with an FDA-approved product. Both medicines may cause thyroid tumors, including cancer: don’t use either if you or a family member has had medullary thyroid carcinoma (MTC) or multiple endocrine neoplasia syndrome type 2 (MEN 2).
Read the full safety information
In studies with rodents, both medicines caused thyroid tumors; it isn’t known whether they cause them in people. Tell your provider if you notice a lump or swelling in your neck, hoarseness, trouble swallowing or shortness of breath. Serious side effects can include inflammation of the pancreas (pancreatitis), gallbladder problems, low blood sugar (especially with insulin or sulfonylureas), kidney problems from dehydration, severe stomach problems, serious allergic reactions, vision changes in people with type 2 diabetes, and a faster heart rate. Tell your care team before any surgery or procedure that uses anesthesia or deep sedation. Don’t combine either medicine with other
Tirzepatide can make birth control pills less effective: switch to a non-oral method, or add a barrier method such as condoms, for 4 weeks after you start and for 4 weeks after each dose increase. Both medicines may harm an unborn baby. Semaglutide should be stopped at least 2 months before a planned pregnancy. Tell your provider right away if you’re pregnant, planning to be, or breastfeeding.
This is a summary, not the complete safety information. Read the full Prescribing Information for FDA-approved tirzepatide (Zepbound) and semaglutide (Wegovy), and talk with your provider. You can report side effects to the FDA at
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Who checked this page
Leads the NexLife clinical team, which medically reviews each of NexLife’s guides, including this one.
Labels and FDA, CDC and NIH guidance read October 27, 2026.
- How we checked. Wegovy comes in pens, prefilled syringes and tablets, not vials, so the steps follow the Wegovy prescribing information (revised June 2026), its Medication Guide and its instructions for use only where they apply to a vial and syringe. Drawing from the vial follows NIH’s MedlinePlus, and multi-dose vials and sharps follow the FDA and the CDC; the insulin rule comes from the Ozempic prescribing information (revised May 2026). We read them all on October 27, 2026. Units are our math: units = mg ÷ mg/mL × 100. We don’t give doses: your prescription sets your units.
- Every fact, sourced. Each price and rule links to the page it comes from.
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4.8 out of 5 on Trustpilot · Medical director Adam Kennah, MD (NPI 1144260043) · LegitScript-certified
Compounded medications aren’t FDA-approved. A provider prescribes one only when it offers a significant difference for you.
- 1Tell us about you
Start online with your health history.
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Online, or by secure video where your state requires a live visit. You’re prescribed only if treatment is right for you.
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Sources
- Wegovy (semaglutide) prescribing information, Medication Guide and Instructions for Use, Novo Nordisk (revised June 2026). Read October 27, 2026.
- Ozempic (semaglutide) prescribing information, Novo Nordisk (revised May 2026). Read October 27, 2026.
- FDA: concerns with unapproved
GLP-1 drugs used for weight loss (updated October 1, 2026). Read October 27, 2026. - FDA: the best way to get rid of used needles and other sharps. Read October 27, 2026.
- CDC: preventing unsafe injection practices (multi-dose vials). Read October 27, 2026.
- MedlinePlus (NIH): subcutaneous (SQ) injections (reviewed October 19, 2025). Read October 27, 2026.
- MedlinePlus (NIH): giving an insulin injection (reviewed July 21, 2024). Read October 27, 2026.
- Kaiser Permanente drug encyclopedia: U-100 insulin syringe, 0.3 mL, half-unit marking. Read October 27, 2026.
- NexLife: pricing, every plan. Checked October 27, 2026.
- NexLife: Price Match Guarantee† terms.